1978-1979 - pharmacology studies in Berlin, 1979-1985 - medical studies at the Free University of Berlin. She had received her professional diploma in 1986. In 1987 she had worked as an AIDS specialist for schools.
In the years 1988-1989 dr Stief was the scientific assistant at the in vitro fertilisation laboratory of the Free University of Berlin. In 1989-1995 she was a doctor at the UKRV (Rudolf Virchow university hospital of Berlin). In 1997 she was appointed doctor for gynaecology and obstetrics. In the years 1995-1999 she was a doctor at the German Red Cross women's hospital in Charlottenburg, Berlin, after which time she co-founded Fertility Center Berlin in 1999.
Dr Stief completed her basic studies in psychosomatic treatment in 1999. In 2000 she was appointed doctor for reproductive medicine and endocrinology.